![]() Necessary cookies are absolutely essential for the website to function properly. Sezgin, M & Sankur, B (2004), “ Survey over Image Thresholding Techniques and Quantitative Performance Evaluation“, Journal of Electronic Imaging 13(1): 146-165.Li, CH & Tam, PKS (1998), “An Iterative Algorithm for Minimum Cross Entropy Thresholding”, Pattern Recognition Letters 18(8): 771-776.Li, CH & Lee, CK (1993), “Minimum Cross Entropy Thresholding”, Pattern Recognition 26(4): 617-625.Otsu, N (1979), “ A threshold selection method from gray-level histograms“, IEEE Trans.Reimplemented in ImageJ by Johannes Schindelin. Huang, L-K & Wang, M-J J (1995), “ Image thresholding by minimizing the measure of fuzziness“, Pattern Recognition 28(1): 41-51.This original plugin was developed for ImageJ as Auto Threshold, its author is Gabriel Landini. Making use of the GenericDialog class, the plugin is macro-recordable.Source code: Documentation Original Plugin In the case of batch processing, if Save Plot is checked, the plugin creates a new folder in the parent directory called “Graphs” and saves an over-sampled plot with the name of the image and threshold method. The X dimension is in the frequency domain and represents pixels -1įRC Curve example using 1/7 Threshold condition ![]() Finally it displays the threshold method used and the intersection of the FRC with the threshold, providing the FIRE number. The Plugin can display a plot of the FRC curve, along with the LOESS smoothed version of the curve. Results Table example from FRC Calculation Plots The column name reflects the calibration of the image and the threshold method selected. ![]() The plugin writes the FIRE (Fourier Image REsolution) number on a Results Table that gets appended as the plugin gets used. The plugin will open one folder, and perform the FRC for each image that has an image in the second folder with the same name. There is also a Batch Option under Plugins › BIOP › Image Analysis › FRC › FRC Calculation (Batch)… You need two images open to perform the FRC. This plugin is available from the PTBIOP Update Site This places it in a “BIOP” Folder in the plugins directory of Fiji/ImageJ Use Direct UseĬall up the plugin using Plugins › BIOP › Image Analysis › FRC › FRC Calculation…. The plugin will always return both the non calibrated values and the calibrated ones as separate columns. We recommend you use a calibrated image so that your results are in calibrated units (microns, millimeters, etc.) directly. Please see the publication van Hell and Schatz, Fourier shell correlation threshold criteria, Journal of Structural Biology 151, 2005 2 for more information on the calculation of these criteria.
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